Project output

• Kulmer ST, Gutmann A, Lemmerer M and Nidetzky B. 2017. Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose. Advanced synthesis & catalysis 2017, 359, 292-301. DOI: 10.1002/adsc.201601078

• Orrego, A. H., Trobo-Maseda, L., Rocha-Martin, J., Guisan, J.M.. 2017. Immobilization-Stabilization of a complex multimeric sucrose synthase from Nitrosomonas europaea. Synthesis of UDP-Glucose. Enzyme and Microbial Technology. 105: 51-58. DOI: 10.1016/j.enzmictec.2017.06.008

• Ortiz-Soto ME, Possiel C, Görl J, Vogel A, Schmiedel R, Seibel J (2017) Impaired coordination of nucleophile and increased hydrophobicity in the +1 subsite shift levansucrase activity towards transfructosylation. Glycobiology (2017) 27 (8): 755-765. DOI: 10.1093/glycob/cwx050

• van den Bergh T, Tamo G, Nobili A, Tao YF, Tan TW, Bornscheuer UT, Kuipers RKP, Vroling B, de Jong RAM, Subramanian K, Schaap PJ, Desmet T, Nidetzky B, Vriend G and Joosten HJ (2017) CorNet: Assigning function to networks of co-evolving residues by automated literature mining. PLoS ONE 12(5): e0176427

• Gutmann A, Lepak A, Diricks M, Desmet T, Nidetzky B (2017) Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP. Biotechnol J. 12(7). doi: 10.1002/biot.201600557.

• Gutmann A1 and Nidetzky B1,2. 2016. Unlocking the Potential of Leloir Glycosyltransferases for Applied Biocatalysis: Efficient Synthesis of Uridine 5′-Diphosphate-Glucose by Sucrose Synthase. Advanced Synthesis & Catalysis 358: 3600-3609. DOI: 10.1002/adsc.201600754

• Schmölzer K, Lemmerer M, Gutmann A, Nidetzky B (2016) Integrated process design for biocatalytic synthesis by a Leloir Glycosyltransferase: UDP-glucose production with sucrose synthase. Biotechnol. Bioeng. 9999: 1–5 DOI: 10.1002/bit.26204

Video abstract of the paper can be found here

• Lemmerer M, Schmölzer K, Gutmann A, Nidetzky B (2016) Downstream Processing of Nucleotide-Diphospho-Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption. Adv. Synth. Catal. DOI: 10.1002/adsc.201600540

• Dewitte G, Walmagh M, Diricks M, Lepak A, Gutmann A, Nidetzky B, Desmet T (2016) Screening of Recombinant Glycosyltransferases Reveals the Broad Acceptor Specificity of Stevia UGT-76G1. J. Biotechnol. 233:49-55 DOI: 10.1016/j.jbiotec.2016.06.034

• Diricks M, Gutmann A, Debacker S, Dewitte G, Nidetzky B, Desmet T (2016) Sequence determinants underlying the nucleotide preference of Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues. PEDS p 1-8 DOI: 10.1093/protein/gzw048

• Schmölzer K, Gutmann A, Diricks M, Desmet T, Nidetzky B (2016) Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development. Biotechnol. Adv. 34(2): 88–111

• Bungaruang L, Gutmann A, Nidetzky B (2016): β-Cyclodextrin Improves Solubility and Enzymatic C-Glucosylation of the Flavonoid Phloretin. Adv. Synth. Catal. 358: 486-493

• Diricks M, De Bruyn F, Van Daele P, Walmagh M, Desmet T (2015): Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes. Appl. Microbiol. Biotechnol. 99(20):8465-8474

• Lepak A, Gutmann A, Kulmer ST, Nidetzky B (2015): Creating a water-soluble resveratrol-based antioxidant by site-selective enzymatic glucosylation. Chembiochem 16(13):1870-1874

• Walmagh M, Zhao R, Desmet T (2015) Trehalose Analogues: Latest insights in properties and biocatalytic production. Int. J. Mol. Sci. 16, 13729-13745

• Gutmann A, Bungaruang L, Weber H, Leypold M, Breinbauerb R, Nidetzky B (2014) Towards the synthesis of glycosylated dihydrochalcone natural products using glycosyltransferase-catalysed cascade reactions. Green Chem. 16, 4417-4425


Background articles

• Desmet T, Soetaert W, Bojarová P, Kren V, Dijkhuizen L, Eastwick-Field V and Schiller A (2012) Enzymatic glycosylation of small molecules: challenging substrates require tailored catalysts. Chemistry: A European Journal, 18: 10786-10801.

• Gutmann A, and Nidetzky B (2012) Switching between O- and C-glycosyltransferase through exchange of active-site motifs. Angewandte Chemie Int. Ed. Engl., 51: 12879-12883.

• Zuccaro A, Götze S, Kneip S, Dersch P and Seibel J (2008) Tailor-made fructooligosaccharides by a combination of substrate and genetic engineering. ChemBioChem, 9:143-149.

• Görl J, Timm M and Seibel J (2012) Mechanism-Oriented Redesign of an Isomaltulose Synthase to an Isomelezitose Synthase by Site-Directed Mutagenesis. ChemBioChem, 13: 149.

• Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM and Fernandez-Lafuente R (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme and Microbial Technology, 40 (6): 1451-1463.

• Kuipers R, Joosten H, van Berkel W, Leferink N, Rooijen E, Ittmann E, van Zimmeren F, Jochens H, Bornscheuer U, Vriend G, dos Santos V and Schaap P (2010) 3DM: systematic analysis of heterogeneous superfamily data to discover protein functionalities. Proteins, 78: 2101-2113.

• Goedl C, Sawangwan T, Mueller M, Schwarz A and Nidetzky B (2008) A high-yielding biocatalytic process for the production of 2-O-(alpha-D-glucopyranosyl)-sn-glycerol, a natural osmolyte and useful moisturizing ingredient. Angew Chem Int Ed Engl., 47(52):10086-10089.

Seventh Framework Programme